PhosLite™ Green

Catalogue Number: 11630-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Enzyme Substrates
Shipping Condition:RT
Unit(s): 1 mg


Description: This water-soluble PhosLite™ Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique properties (such as an extremely large Stokes shift and high photostability), making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.

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Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months