Catalogue Number: 11A4-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD11a*PE |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | OFYS |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | FC |
Description: CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
Itgal
3683
Protein A/G Purified
P20701
Monoclonal
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Product should be stored at 4-8C. DO NOT FREEZE