Catalogue Number: 11B3-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD11b Biotin |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | F6.2 |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | FC |
Description: (Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes.
ITGAM
3684
P11215
Protein A/G Purified
Monoclonal
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Product should be stored at 4-8C. DO NOT FREEZE