Mouse anti Human CD11b, conjugated with Biotin

Catalogue Number: 11B3-NMB

Manufacturer:Nordic MuBio
Type:Monoclonal Primary Antibody - Conjugated
Alias:CD11b Biotin
Shipping Condition:RT
Unit(s): 100 tests
Host name: Mouse
Clone: F6.2
Isotype: IgG2a
Immunogen:
Application: FC

Description

Description: (Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes.

Additional Text

Gene Name

ITGAM

Gene ID

3684

Uniprot ID

P11215

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE