Anti SARS-CoV-2 Nucleocapsid Rabbit mAb (Clone:84D7) Biotin Conjugate
Catalogue Number: 12-4003-B-ABO
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12-4003-B Image
12-4003-B Image
Figure 1: Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Anti-SARS-CoV-2 Nucleocapsid Rabbit mAb Clone 84D7 Biotin Conjugate (Cat# 12-4003-B) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.
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Figure 2: A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: Clone 75G5a (Cat# 12-4001) as a capture antibody and Clone 84D7-biotin (Cat# 12-4003-B) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibody clones 75G5a and 84D7-biotin detected COVID-19 NP antigen at very high sensitivity, as low as 25 pg/mL (2.5 pg).
| Manufacturer: | Abeomics |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Rabbit |
| Clone: | 84D7 |
| Isotype: | IgG1, kappa |
| Immunogen: | SARS-CoV-2 nucleoprotein (NP) specific peptide. |
| Application: | ELISA |
Description
Description: SARS-CoV-2 Nucleocapsid antibodies, which include clones 84D7, 75G5a, 84C4a and 85C1, can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. These antibodies do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.
Additional Text
Gene Name
N
Purification
Protein A purified
Antibody Clonality
Monoclonal
Gene ID
43740575
Uniprot ID
P0DTC9
Application Notes
Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
12-4003-B Datasheet
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