ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
Catalogue Number: 1230-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Conjugation Kit |
| Shipping Condition: | RT |
| Unit(s): | 2 labelings |
Description
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. iFluor® 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor® 594 family has the spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor® 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594.Compared to Texas Red®, iFluor® 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor® 594 tandem has better FRET efficiency.ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 594 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 594 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 594 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 594 SE.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
1230 Protocol
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