BG-SiR Conjugate
Catalogue Number: 12559-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 24 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Site-specific labeling of cellular proteins with chemical probes is a powerful tool for studying protein functions in living cells. The SNAP-tag is a modified form of the DNA repair enzyme, human O6-alkylguanine-DNA-alkyltransferase (AGT) and undergoes a self-labeling reaction to form a covalent bond with O6-benzylguanine (BG) derivatives. BG-SiR conjugate is a fluorescent SNAP substrate that can be used to label SNAP tag-fused target proteins. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. SNAP-SiR substrate enables the specific, covalent attachment of SiR fluorophore to a protein of interest. SiR has a similar fluorescence spectrum to Cy5 or Alexa Fluor 647. The labeled proteins can be imaged with Cy5 filter set.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|24 months
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