Amplite® Colorimetric Beta-Galactosidase Assay Kit

Catalogue Number: 12604-AAT

Manufacturer:	AAT Bioquest
Type:	Enzyme Assays
Shipping Condition:	Dry Ice
Unit(s):	200 Tests

Overview
Datasheets

Description

Description: E. coli β-galactosidase is a 464 kD tetramer. Each unit of β-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, β-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of β-galactosidase per cell. This kit uses a chromogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The light yellow substate generates a strongly red product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite™Colorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used for screening galactosidase inhibitors or inducers.

Additional Text

Storage Note

Multiple;|6 months

12604 Protocol

12604 MSDS

Price

£328.00

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