HIS Lite™ OG488-Tris NTA-Ni Complex
Catalogue Number: 12615-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Probes |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
Description
Description: Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. The transition metal ions (e.g., Ni ion)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time. This OG488-tris-NTA compound has been reported to be an sensitive fluorescent probe for detecting polyhistidine-labeled proteins in cells, solution and solid surfaces.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
12615 MSDS
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