Rhodamine Red-X-conjugated AffiniPure Fab Fragment Goat Anti-Alpaca IgG, VHH domain ( min X Bov,Hu,Ms,Rb,Rat Sr Prot)

Catalogue Number: 128-297-230-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.25 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgG, VHH domain

Overview
Datasheets

Description

Description: Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on antigen-binding assay, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3; and with the VHH domain of llama IgG, subclasses 2 and 3. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Alpaca IgG, VHH domain secondary antibody, conjugated to Rhodamine Red™-X (RRX). This is an affinity purified Fab Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Human, Mouse, Rabbit and Rat serum proteins.

Application Notes

1:50 - 1:200 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Storage Note

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Guide to Immunoglobulins Structure and Function