Histone H3K4me2 Antibody, SNAP-Certified™ for CUT&RUN
Catalogue Number: 13-0027-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 dimethylated at lysine 4 |
| Application: | ICC, WB, CUT&RUN |
Description
Description: This antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in CUT&RUN. This requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figure 1). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k starting cells (Figures 2-4). This antibody targets histone H3 dimethylated at lysine 4, which is enriched in promoters of transcriptionally active genes and genes primed for expression during cell development [1].
Additional Text
Purification
Affinity Purified
Modification
Dimethylated, Lys4
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN 0.5 µg per reaction Immunocytochemistry 1 - 2 µg/mL Western Blot 0.02 - 0.2 µg/mL
Storage Note
Stable for 1 year at -20°C from date of receipt
13-0027 Datasheet
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