CUTANA™ IgG Negative Control Antibody for CUT&RUN and CUT&Tag

Catalogue Number: 13-0042-EPC

Manufacturer:EpiCypher
Shelf Life:12 months
Type:Isotype control - Unconjugated
Host Cell:Rabbit|Antibody
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug
Host name: Rabbit
Clone:
Isotype:
Immunogen: None
Application: CUT&RUN, CUT&Tag

Description

Description: Cleavage Under Targets & Release Using Nuclease (CUT&RUN) and Cleavage Under Targets and Tagmentation (CUT&Tag) are next generation assays for genomic mapping of chromatin features to replace Chromatin Immunoprecipitation (ChIP-seq) [1]. Unlike in ChIP-seq, where baseline signal is established by sequencing the fragmented input chromatin, reactions performed with this rabbit IgG antibody are used to determine background signal arising from non-specific MNase digestion and Tn5 tagmentation. Pair with the H3K4me3 positive control antibody (EpiCypher 13-0041) for a well-controlled experiment.

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN: 0.5 µg per reaction CUT&Tag: 0.5 µg per reaction

Storage Note

Stable for 1 year at 4°C from date of receipt