Rabbit IgG Antibody, CUTANA CUT&RUN Negative Control

Catalogue Number: 13-0042-EPC

Manufacturer:EpiCypher
Shelf Life:12 months
Type:Isotype control - Unconjugated
Shipping Condition:Dry Ice
Storage Condition:2-8°C
Unit(s): 100 ug
Host name: Rabbit
Clone:
Isotype:
Immunogen: None
Application: CUT&RUN

Description

Description: This Rabbit IgG Antibody can be used to control for non-specific background signal in CUT&RUN and other applications. A positive control antibody is also available (H3K4me3, EpiCypher Catalog No. 13-0041).

Additional Text

Antibody Clonality

Monoclonal

Application Notes

Prior to use, prepare a 1:10 working stock dilution in CUT&RUN Antibody Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.01% Digitonin, 2 mM EDTA). Store at 4°C for 1 month. For each CUT&RUN assay, dilute 1:100 from the working stock. A typical yield for a CUT&RUN assay using 500,000 K562 cells is 2-5 ng DNA.