Histone H3K27ac Antibody, SNAP-ChIP® Certified

Catalogue Number: 13-0045-EPC

Shelf Life:12 months
Physical state:Aff. Pur. IgG
Type:ChIP Validated Antibodies
Host Cell:Mouse|Antibody
Shipping Condition:Blue Ice
Unit(s): 50 ug
Host name: Mouse
Immunogen: A synthetic peptide corresponding to histone H3 acetylated at lysine 27
Application: ICC, IF, ChIP


Description: This antibody meets EpiCypher's SNAP-ChIP® Certified criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody binds to H3K27ac and no significant cross reactivity with other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat Panel (EpiCypher 19-3001) is detected. Antibody binding to H3K27ac in the context of phosphorylation at S28 (H3K27acS28ph) is inhibited to varying degrees in ChIP (Figure 1).

Additional Text


Affinity Purified


Acetylated, Lys27

Antibody Clonality


Application Notes

Chromatin Immunoprecipitation 2 - 5 µg per 5 µg chromatin Immunocytochemistry/Immunofluorescence 1:500

Storage Note

Stable for 1 year at -20°C from date of receipt