Histone H3K18acyl Antibody, SNAP-ChIP® Certified
Catalogue Number: 13-0050-EPC
| Manufacturer: | EpiCypher |
| Physical state: | Aff. Pur. IgG |
| Type: | ChIP Validated Antibodies |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 acetylated at lysine 18. |
| Application: | ICC, IF, IP, ChIP, WB, IHC |
Description
Description: This antibody meets EpiCypher's "SNAP-ChIP® Certified" criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input) based on technology originating from Grzybowski et al. [1] and profiling standards from Shah et al. [2]. This antibody reacts to H3K18 acetylation as well as extended acyl states (butyrylation, bu; crotonylation, cr) when present alone and in combination (H3K4,9,14,18ac). No cross reactivity to other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel (EpiCypher 19-3001) is detected.
Additional Text
Purification
Affinity Purified
Modification
Acetylated, Lys18
Antibody Clonality
Polyclonal
Application Notes
ChIP: 2 - 5 µg per 106 cells IHC: 1:100 - 1:1,000 ICC/IF: 1:100 - 1:1,000 IP: 1:100 - 1:500 WB: 1:1,000 - 1:10,000
Storage Note
Store at 4°C after thawing. Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. Stable for 1 year at -20°C from date of receipt.
Preparation
100 µL of protein in a screw cap plastic vial|Aff. Pur. IgG
13-0050 Datasheet
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