H3K27me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag
Catalogue Number: 13-0055-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 2084-1G5 |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 trimethylated at lysine 27 |
| Application: | CUT&RUN, CUT&Tag |
Description
Description: This H3K27me3 (histone H3 lysine 27 trimethyl) antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and CUT&Tag applications. This requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figures 1 and 4). High target efficiency is confirmed by consistent genomic enrichment at varying cell inputs: 500k and 50k cells in CUT&RUN (Figures 2-3); 100k and 10k cells in CUT&Tag (Figures 5-6). High efficiency antibodies display similar peak structures at representative loci (Figures 3 and 6) and highly conserved genome-wide signal (Figures 2 and 5) even at reduced cell numbers. H3K27me3 is associated with repressed genes [1] and is anti-correlated with H3K4me3, a marker of active gene transcription enriched at transcription start sites (Figures 2 and 5).
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN: 0.5 µg per reaction CUT&Tag: 0.5 µg per reaction
Storage Note
Stable for 1 year at 4°C from date of receipt
13-0055 Datasheet
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