H3K27ac Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag
Catalogue Number: 13-0059-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 2114-3E4 |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 acetylated at lysine 27 |
| Application: | CUT&RUN, CUT&Tag |
Description
Description: This H3K27ac (histone H3 acetylated at lysine 27) antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and CUT&Tag applications. This requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-AcylStat Panel of spike-in controls (EpiCypher RD193002, Figures 1 and 4). High target efficiency is confirmed by consistent genomic enrichment at varying cell inputs: 500k and 50k cells in CUT&RUN (Figures 2-3); 100k and 10k cells in CUT&Tag (Figures 5-6). High efficiency antibodies display similar peak structures (Figures 3 and 6) and highly conserved genome-wide signal (Figures 2 and 5) even at reduced cell numbers. H3K27ac is associated with gene activation and is enriched at active enhancers and promoters [1].
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN: 0.5 µg per reaction CUT&Tag: 0.5 µg per reaction
Storage Note
Stable for 1 year at 4°C from date of receipt
Preparation
100 µg in a screw cap plastic vial|Aff. Pur. IgG
13-0059 Datasheet
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