H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag
Catalogue Number: 13-0060-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 2909-3D7 |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 trimethylated at lysine 4 |
| Application: | CUT&RUN, CUT&Tag |
Description
Description: This H3K4me3 (histone H3 trimethylated at lysine 4) antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and CUT&Tag applications. This requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figures 1 and 4). High target efficiency is confirmed by consistent genomic enrichment at varying cell inputs: 500k and 50k cells in CUT&RUN (Figures 2-3); 100k and 10k cells in CUT&Tag (Figures 5-6). High efficiency antibodies display similar peak structures (Figures 3 and 6) and highly conserved genome-wide signal (Figures 2 and 5) even at reduced cell numbers. This antibody targets histone H3K4me3, which is enriched at active promoters near transcription start sites (TSS) and promotes gene activation.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN: 0.5 µg per reaction CUT&Tag: 0.5 µg per reaction
Storage Note
Stable for 1 year at 4°C from date of receipt
Preparation
100 µg in a screw cap plastic vial|Aff. Pur. IgG
13-0060 Datasheet
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