H3.3 Antibody, SNAP-Certified™ for CUT&RUN
Catalogue Number: 13-0061-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | 2893-4D5 |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to the N-terminal tail (including amino acid 31) of histone H3.3 |
| Application: | CUT&RUN |
Description
Description: H3.3 Antibody, SNAP-Certified™ for CUT&RUN is an antibody to histone variant H3.3. H3.3 is enriched in promoters and enhancers of active genes as well as transcriptionally inactive heterochromatin, reflecting the diverse functions of this unique histone variant [1]. The antibody is highly specific for H3.3 without cross-reacting with canonical H3.1. Binding is not impacted by methylation or mutations at amino acids H3.3K27 or H3.3K36 (Figure 1). H3.3 differs from canonical H3.1 at just five amino acids (31, 87, 89, 90, 96). Most of these changes are located in the inaccessible histone core, making them unfit epitopes for chromatin mapping experiments. The SNAP-Certified™ H3.3 antibody specifically interacts with amino acid 31, where a serine is substituted for alanine, allowing clear distinction between H3.3 and H3.1 in chromatin mapping experiments. The H3.3 antibody meets EpiCypher's SNAP-Certified™ criteria for specificity and efficiency in CUT&RUN. This requires <20% cross-reactivity to other histone variants, determined using SNAP-CUTANA™ Spike-in Controls (Figure 2). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k cells (Figure 3). Note: This antibody is sensitive to dithiothreitol (DTT).
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Storage Note
Stable for 1 year at 4°C from date of receipt
Application Notes
CUT&RUN: 0.5 µg per reaction Note: Not recommended for use with buffers containing reducing agents.
Preparation
100 µg in a screw cap plastic vial|Aff. Pur. IgG
13-0061 Datasheet
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