BRM/SMARCA2 CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2006-EPC

Manufacturer:EpiCypher
Shelf Life:12 months
Physical state:Aff. Pur. IgG
Type:Polyclonal Primary Antibody - Unconjugated
Host Cell:Rabbit|Antibody
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ul
Host name: Rabbit
Clone:
Isotype:
Immunogen: Between amino acids 1 and 50
Application: IP, WB, CUT&RUN

Description

Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. BRM/SMARCA2 antibody produces CUT&RUN peaks above background (Figure 1) localized to gene transcription start sites (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the SWI/SNF chromatin remodeler complex [1].

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Western Blot 1:2,000 - 1:10,000 Immunoprecipitation 2 - 5 µg/mg lysate

Storage Note

Stable for 1 year at 4°C from date of receipt