Catalogue Number: 13-2009-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Preservative: | 0.09% sodium azide. |
| Ph: | 7 to 8 |
| Physical state: | Aff. Pur. IgG |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Between amino acids 1950 and 2000 |
| Application: | IP, IHC, CUT&RUN |
Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CHD3 is an understudied member of the nucleosome remodeling and deacetylase (NuRD) complex. CHD3 antibody produces CUT&RUN peaks above background (Figure 1) within gene promoters, intergenic regions, and introns (Figures 1-2).
Affinity Purified
Polyclonal
CUT&RUN 0.5 µg per reaction Immunoprecipitation 2 - 5 µg/mg lysate Immunohistochemistry 1:500 - 1:2,000 Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Stable for 1 year at 4°C from date of receipt
100 µL of protein in a screw cap plastic vial|Aff. Pur. IgG