Estrogen Receptor Alpha (N-Terminal) CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2011-EPC

Manufacturer:	EpiCypher
Shelf Life:	12 months
Preservative:	0.09% sodium azide
Ph:	7-8
Physical state:	Aff. Pur. IgG
Type:	Polyclonal Primary Antibody - Unconjugated
Host Cell:	Rabbit\|Antibody
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Between amino acids 1 and 50
Application:	IP, IHC, CUT&RUN

Overview
Datasheets

Description

Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. Estrogen Receptor Alpha N-terminal (ER alpha N-term) antibody shows CUT&RUN peaks in response to estradiol stimulation (Figure 1) that overlap with known estrogen response element (ERE) binding motifs (Figure 2). Overlap is further observed with peaks from an antibody to a different ER alpha epitope (C-term) and NCOA3 (SRC3), which co-activates ER-mediated transcription [1] (Figure 2).

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Immunoprecipitation 3 - 5 µg/mg lysate Immunohistochemistry 1:1,000 - 1:5,000 Epitope retrieval with citrate buffer pH 9.0 is recommended for FFPE tissue sections

Storage Note

Stable for 1 year at 4°C from date of receipt

13-2011 Datasheet

Price

£432.00

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