TP53/p53 CUTANA™ CUT&RUN Antibody
Catalogue Number: 13-2015-EPC
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Figure 1: TP53/p53 peaks in CUT&RUN. CUT&RUN was performed as described above. Peaks were called using MACS2. Heatmaps show TP53/p53 peaks relative to IgG negative control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal (left). The number of peaks that fall into distinct classes of functionally annotated genomic regions are shown (right).
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Figure 2: TP53/p53 transcription factor binding motif analysis in CUT&RUN. NFY (CCAAT)/Promoter/Homer consensus motif, represented as a sequence logo position weight matrix, was the top called motif significantly enriched under TP53/p53 CUT&RUN peaks (left). The number of TP53/p53 peaks containing the consensus motif from the left panel is represented by a Venn Diagram (right).
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Figure 3: TP53/p53 CUT&RUN representative browser tracks. CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci of the top called peaks are shown.
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Figure 4: Immunoprecipitation data. EpiCypher TP53/p53 antibody (6 μg/reaction) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HEK293T cells. A negative control IgG antibody and positive control antibodies targeting the same epitope (Bethyl Laboratories) were also used to demonstrate specificity of the IP. For blotting immunoprecipitates, EpiCypher TP53/p53 antibody was used at a 1:2,500 dilution.
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Figure 5: Western blot data. Western analysis of TP53/p53 in whole cell extracts from HeLa, HEK293T, and Jurkat cells. Fifty micrograms of lysate was resolved via SDS-PAGE and detected with a 1:10,000 dilution of TP53/p53 antibody.
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Preservative: | 0.09% sodium azide |
| Ph: | 7-8 |
| Physical state: | Aff. Pur. IgG |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Between amino acids 325 and 375 |
| Application: | IP, WB, CUT&RUN |
Description
Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. TP53/p53 acts as a tumor suppressor by promoting growth arrest or apoptosis and is mutated in over 50% of cancers. It forms a complex with NF-Y, which binds to CCAAT box-containing promoters [1].TP53/p53 antibody produces CUT&RUN peaks above background primarily in promoter regions (Figure 1) that are enriched with known TP53/p53 DNA-binding motifs (Figure 2).
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Application Notes
CUT&RUN 0.5 µg per reaction Western Blot 1:2,000 - 1:10,000 Immunoprecipitation 2-10 µg/mg lysate
Storage Note
Stable for 1 year at 4°C from date of receipt
13-2015 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations