SP1 CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2024-EPC

Manufacturer:	EpiCypher
Shelf Life:	12 months
Preservative:	0.09% sodium azide
Ph:	7-8
Physical state:	Aff. Pur. IgG
Type:	Polyclonal Primary Antibody - Unconjugated
Host Cell:	Rabbit\|Antibody
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Between amino acids 750 and 785
Application:	IP, WB, IHC, CUT&RUN

Overview
Datasheets

Description

Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SP1 is the original member of the Sp1-like family of zinc-finger transcription factors. It binds GC-rich motifs with high affinity [1] and is involved in expressing genes related to cell growth, cell-cycle progression, survival, and tumorigenesis [2]. SP1 antibody produces CUT&RUN peaks above background primarily in intronic and promoter regions (Figure 1) that overlap with known SP1 DNA-binding motifs (Figure 2).

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Immunoprecipitation 2-10 µg/mg lysate Immunohistochemistry 1:2000-1:10,000 Western Blot 1:2,000 - 1:10,000 Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Storage Note

Stable for 1 year at 4°C from date of receipt

Preparation

100 µL of protein in a screw cap plastic vial|Aff. Pur. IgG

13-2024 Datasheet

Price

£459.00

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