DYKDDDDK Tag CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2031-EPC

Manufacturer:	EpiCypher
Shelf Life:	12 months
Physical state:	Aff. Pur. IgG
Type:	Monoclonal Primary Antibody - Unconjugated
Host Cell:	Rabbit\|Antibody
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	A synthetic peptide sequence (DYKDDDDK)
Application:	ELISA, ICC, IP, WB, CUT&RUN

Overview
Datasheets

Description

Description: DYKDDDDK antibody is useful for studies utilizing FLAG®*-tagged target proteins. This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target. DYKDDDDK Tag antibody produces CUT&RUN peaks (Figures 2-3) that overlap with GATA3 in breast cancer cells expressing 3xFLAG-tagged GATA3 transcription factor [1]** *FLAG® is a registered trademark of Merck KGaA, Darmstadt, Germany. **Thanks to Dr. Takaku (UND) for 3xFLAG-GATA3-3xHA MDA-MB-231 cells.

Additional Text

Purification

Affinity Purified

Antibody Clonality

Monoclonal

Storage Note

Stable for 1 year at 4°C from date of receipt

Application Notes

CUT&RUN 0.05 µg per reaction (0.5 µL of 100 µg/mL antibody) Enzyme-Linked Immunosorbent Assay 1:1,000 - 1:75,000; for coating plates 1:100 - 1:500 Western Blot 1:1,000 Immunoprecipitation 20 µL/mg lysate Immunocytochemistry 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections

Preparation

100 µL of protein in a screw cap plastic vial|Aff. Pur. IgG

13-2031 Datasheet

Price

£476.00

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