SUPT5H (SPT5, SPT5H, Transcription Elongation Factor SPT5, DRB Sensitivity-inducing Factor 160kD Subunit, DRB Sensitivity-inducing Factor Large Subunit, Tat-cotransactivator 1 Protein, FLJ34157) (MaxLight 490)

Catalogue Number: 134102-ML490-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Type:	Monoclonal Primary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	3F1
Isotype:	IgG2b, kappa
Immunogen:	Partial recombinant corresponding to aa981-1088 from human SUPT5H (AAH24203) with GST tag. MW of the GST tag alone is 26kD.
Application:	IF, WB, IHC, FLISA

Overview
Datasheets

Description

Description: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Additional Text

Specificity

Recognizes human SUPT5H.

Purification

Protein A purified

Accession Number

BC024203

Antibody Clonality

Monoclonal

134102-ML490 Datasheet

Price

£896.00

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