Amplite® Universal Fluorimetric Protease Activity Assay Kit *Red Fluorescence*
Catalogue Number: 13501-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Enzyme Assays |
| Shipping Condition: | Dry Ice |
| Unit(s): | 500 Tests |
Description
Description: Monitoring of various protease activities has become a routine task for many biological laboratories. Our Amplite™ Universal Fluorimetric Protease Activity Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. The kits use fluorescent casein conjugates that are proven to be a generic substrate for a broad spectrum of proteases. In the intact substrate, casein is heavily labeled with a fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The kits provide all the essential components with an optimized “mix & read” protocol that can be easily automated to HTS instruments.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
13501 Protocol
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