Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
Catalogue Number: 13502-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Enzyme Assays |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 Tests |
Description
Description: Caspases play important roles in apoptosis and cell signaling. The activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 is also identified as a drug-screening target. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This Amplite™ Caspase-3 Assay Kit uses Ac-DEVD-AMC as fluorogenic indicator for assaying caspase-3 activity. AMC-derived caspase substrates are widely used for fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm. This kit can be used to continuously measure the activities of caspase-3 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer.
Additional Text
Storage Note
Multiple;|6 months
13502 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations