Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Catalogue Number: 13830-AAT

Manufacturer:	AAT Bioquest
Type:	Detection Kit
Shipping Condition:	Dry Ice
Unit(s):	200 Tests

Overview
Datasheets

Description

Description: Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop. The two main ketone bodies are beta-hydroxybutyrate (beta-HB) and acetoacetate (AcAc), while acetone is the third abundant ketone body. Normally these two predominant ketone bodies are present in small amounts in the blood during fasting (low food intake) and prolonged exercise. In patients who have diabetes, alcohol or salicylate poisoning, hormone deficiency, childhood hypoglycemia and other acute disease states, large quantities of ketone bodies are found in the blood. The over-production and accumulation of ketone bodies in the blood (ketosis) can lead to pathological metabolic acidosis (ketoacidosis). In extreme cases, ketoacidosis can be fatal. Blood ketone testing methods that quantify beta-HB, the predominant ketone body in the blood (approximately 75%) have been used for diagnosing and monitoring treatment of ketoacidosis. Amplite® Colorimetric Beta-Hydroxybutyrate Assay Kit offers a sensitive colorimetric assay for measuring beta-HB levels in biological samples. This assay is based on an enzyme coupled reaction of beta-HB, in which the NADH product can be specifically monitored by a NADH sensor. The signal can be measured by an absorbance microplate reader with the OD ratio at the wavelength of 570 nm to 610 nm. With this Amplite® Colorimetric Beta-Hydroxybutyrate Assay Kit, we were able to detect as low as 4 µM beta-HB in a 100 µL reaction volume.

Additional Text

Storage Note

Multiple;|6 months

13830 MSDS

13830 Protocol

Price

£399.00

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