CD45RO (Leukocyte Common Antigen, LCA) (MaxLight 490)

Catalogue Number: 138519-AF-ML490-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	B220\|CD45\|CD45 antigen\|CD45R\|GP180\|LCA\|L-CA\|LY5\|protein tyrosine phosphatase, receptor type, c polypeptide\|receptor-type tyrosine-protein phosphatase C\|T200\|T200 glycoprotein\|T200 leukocyte common antigen
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	UCHL-1
Isotype:	IgG2a, kappa
Immunogen:	Interleukin-2-dependent human T lymphocytes
Application:	IF, IP, WB, IHC, FLISA

Overview
Datasheets

Description

Description: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.

Additional Text

Gene Name

PTPRC

Gene ID

5788

Uniprot ID

P08575

Accession Number

NP_001254727

Specificity

Recognizes human CD45RO. Species Crossreactivity: chimpanzee and common marmoset.

Purification

Protein G purified

Antibody Clonality

Monoclonal

138519-AF-ML490 Datasheet

Price

£883.00

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