Catalogue Number: 14-1048-EPC

Shelf Life:6 months
Type:DNA Sequencing Kits
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 48 reactions
Sample type:
Sample size:


Description: The CUTANA™ ChIC/CUT&RUN Kit enables streamlined chromatin profiling of histone post-translational modifications (PTMs) and chromatin associated proteins. The CUT&RUN Kit Version 4 (v4) now uses SPRI magnetic beads for DNA purification instead of DNA spin columns, enabling multi-channel pipetting throughout for increased throughput and reproducibility. Positive (H3K4me3) and negative (IgG) control antibodies are included to pair with SNAP-CUTANA™ spike-in controls for assay optimization and continuous assay monitoring (Figure 2). E. coli DNA is included for data normalization. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 5,000 cells. The inclusion of controls, as well as compatibility with diverse target types, sample inputs, and low cell numbers, make this kit the go-to solution for chromatin mapping experiments.

Additional Text

OPEN KIT IMMEDIATELY and store components at room temperature, 4°C, and -20°C as indicated (see User Manual corresponding to Kit Version 4). Stable for 6 months upon date of receipt.

Application Notes

See User Manual corresponding to Kit Version 4. This kit is not compatible with previous user manuals. Version 4 contains SPRI magnetic beads for DNA clean up, while previous versions used DNA spin columns.