Anti-Neurofilament NF-L Antibody
Catalogue Number: 1453-NFL-PPS
1453-NFL Image
1453-NFL Image
1453-NFL Image
1453-NFL Image
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody (cat. 1453-NFL, red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.
1453-NFL Image
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN( cat. 583-FOX3 , 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
1453-NFL Image
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
| Manufacturer: | Phosphosolutions |
| Preservative: | 10 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Preparation of bovine spinal cord NF-L. |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
NEFL
Molecular Weight
68
Antibody Clonality
Polyclonal
Short Description
Our Anti-Neurofilament NF-L primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is total IgY fraction. It is great for use in WB, IHC, ICC.
Specificity
68 kDa neurofilament protein antibody, 68kDa Neurofilament antibody, 68kDa neurofilament protein antibody, CMT1F antibody, CMT2E antibody, FLJ53642 antibody, Light molecular weight neurofilament protein antibody, NEFL antibody, Neurofilament light antibody, Neurofilament light polypeptide 68kDa antibody, Neurofilament light polypeptide antibody, Neurofilament protein, light chain antibody, Neurofilament subunit NF L antibody, Neurofilament triplet L protein antibody, NF-L antibody, NF68 antibody, NFL antibody, NFL_HUMAN antibody
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Uniprot ID
P02548, P07196
Gene ID
281348; 4747
1453-NFL Datasheet
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