Histone H3.3, Recombinant Human
Catalogue Number: 15-0312-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Physical state: | Lyophilized |
| Type: | DNA Binding Proteins |
| Host Cell: | E.coli |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) expressed in E. coli and purified by FPLC. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). H3.3-H4 associates in vivo with the HIRA chaperone complex.
Additional Text
Uniprot ID
P84243
Gene ID
3020; 3021
Protein Tag
Untagged
Application Notes
Recombinant histone H3.3 is suitable for enzyme assays and nucleosome reconstitution. Reconstitute with distilled water prior to usage.
Gene Name
H3-3A, H3-3B
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Preparation
lyophilized|100 µg lyophilized protein in a screw cap plastic vial
15-0312 Datasheet
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