Catalogue Number: 15-1014-EPC
|Shelf Life:||6 months|
|Host Cell:||Sf9 cells|
|Shipping Condition:||Dry Ice|
Description: Full length recombinant human SMARCA4 (BRG1) Remodeling Enzyme produced in SF9 cells. SMARCA4 is a crucial component of the SWI/SNF complex. It is an ATP-dependent chromatin remodeling enzyme that regulates nucleosome spacing.
This product is sufficient to perform 100 remodeling reactions using EpiDyne-FRET substrate (Catalog No. 16-4201). A single reaction is defined as 10 µl containing [20 nM SMARCA4/ 20 nM EpiDyne-FRET/ 2.5 mM rATP] and remodels to completion in <20 minutes. 5x SMARCA Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, make single-use aliquots when first thawed.