CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows

Catalogue Number: 15-1016-EPC

Manufacturer:EpiCypher
Shelf Life:12 months
Molecular Weight:44kDa
Ph:7.5
Type:Nucleases
Host Cell:E. coli|S. aureus
Shipping Condition:Blue Ice
Unit(s): 50 reactions

Description

Description: CUTANA™ pAG-MNase is the key reagent for performing Chromatin Immunocleavage (ChIC) [1] and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) [2,3]. As a fusion of Proteins A and G to Micrococcal Nuclease, CUTANA pAG-MNase is compatible with target antibodies from various host species and is highly purified to remove contaminating E. coli DNA, which can complicate analysis at low cell numbers. This enzyme enables efficient mapping of chromatin features in ChIC/CUT&RUN, allowing for significant improvements in signal to noise and sequencing depth compared to ChIP-seq.

Additional Text

Protein Tag

6xHIS Tag

Application Notes

Add 2.5 µL of the supplied enzyme to a 50 µL CUT&RUN reaction (20X dilution). For more, see epicypher.com/protocols.

Storage Note

Stable for one year at -20°C from date of receipt