SMARCA5 Chromatin Remodeling Enzyme

Catalogue Number: 15-1024-EPC

Manufacturer:	EpiCypher
Shelf Life:	6 months
Type:	Modifying Enzymes
Host Cell:	SF9 cells\|Human
Shipping Condition:	Dry Ice
Unit(s):	100 reactions
Host name:	Human
Clone:
Isotype:
Immunogen:

Overview
Datasheets

Description

Description: Full length recombinant human SMARCA5 Remodeling Enzyme. SMARCA5 is a core ATPase subunit of the ATP-dependent chromatin remodeling ISWI complexes that regulate nucleosome spacing to control DNA accessibility [1,2]. SMARCA5 containing complexes exhibit substrate preference and distinct remodeling directionalities with different regulatory subunits [3]. Mis-regulation of SMARCA5 is linked with multiple human cancers including leukemia, breast, lung, and gastric cancers [4]. SMARCA5 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.

Additional Text

Protein Tag

FLAG™-6xHIS Tag

Application Notes

This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET Substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing 10 nM SMARCA5 (concentration is lot-specific), 20 nM EpiDyne®-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014; 100 mM Tris HCl pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). See the EpiDyne®-FRET Technical Note (epicypher.com/resources/technical-notes) or contact techsupport@epicypher.com for more information.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

Preparation

100 reactions frozen aqueous solution in a screw cap plastic vial

15-1024 Datasheet

Price

£354.00

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