Lipopolysaccharide-LPS from Ecoli 0111:B4, Ion-Exchange Chromatography,TLR4 ligand
Catalogue Number: 15-1030-ABO
15-1030 Image
15-1030 Image
Fig-1: Induction of TNF-alpha promoter activity mediated by LPS-stimulated TLR4. TNF-alpha Leeporter™ Luciferase Reporter-RAW264.7 Cells (Abeomics, Cat. No. 14-106ACL) were treated with different concentrations of LPS (15-1030) for 16 h, and then luciferase activity was measured.
| Manufacturer: | Abeomics |
| Type: | Protein Ligand |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg, 5 mg |
| Application: | FA |
Description
Description: Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Additional Text
Application Notes
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80°C. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Purification
Ion Exchange
15-1030 Datasheet
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