CUTANA™️Hia5 for Fiber-seq

Catalogue Number: 15-1032-EPC

Manufacturer:	EpiCypher
Shelf Life:	6 months
Molecular Weight:	33.8 kDa
Type:	Transferases
Host Cell:	E. Coli
Shipping Condition:	Blue Ice
Storage Condition:	-80°C
Unit(s):	8 Reactions, 24 Reactions
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Overview
Datasheets

Description

Description: CUTANA™ Hia5 is the key enzyme for Fiber-seq [1], a multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation, and genetic variation at single-molecule resolution. By layering chromatin accessibility on top of standard LRS workflows, Fiber-seq uniquely links epigenetic information (DNA methylation) and complex genetic architecture (e.g., structural variants and haplotype phasing) to chromatin states within the same molecules of DNA.

Hia5 is a DNA adenine methyltransferase (MTase) that uses S-adenosylmethionine (SAM) to catalyze methylation at the N6 position of adenine, producing N6-methyladenine (6mA). Fiber-seq leverages the nonspecific activity of Hia5 to methylate adenines within accessible chromatin regions in situ. This records chromatin accessibility information onto native DNA while preserving endogenous methylation (5mC). Hia5-modified DNA is fully compatible with LRS on both Pacific Biosciences® (PacBio® HiFi Sequencing) and Oxford Nanopore Technologies® (ONT® Nanopore Sequencing) platforms.

Key Advantages

Multiomic insights in one assay – Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants, consolidating what typically requires three or more separate assays into one long-read sequencing assay.

Highest activity 6mA-MTase – Faster labeling via Hia5 enables higher resolution of accessible DNA, with demonstrated utility in protein footprinting applications to resolve transcription factor binding and nucleosome positioning [2-4].

Access complex genomic regions – Profile chromatin accessibility in repetitive and structurally complex regions such as transposable elements, centromeres, telomeres, segmental duplications, and chromosomal rearrangements.

Easy workflow integration – Fiber-seq can be performed in <2 hours directly upstream of routine long-read sequencing library preparation.

Resolve chromatin state heterogeneity – PCR-free approach leverages long-read sequencing to directly profile individual DNA molecules, uncovering chromatin structure heterogeneity in cell populations often missed by short-read techniques.

Background References:

[1] Stergachis et al. Science (2020). PMID: 32587015

[2] Tullius et al. Mol. Cell (2024). PMID: 39191261

[3] Dubocanin et al. Cell Genom. (2025). PMID: 40147439

[4] Vollger et al. bioRxiv (2024). DOI: 10.1101/2024.06.14.599122

[5] Jha et al. Genome Res. (2024). PMID: 38849157

15-1032 Technical datasheet (8rxns)

15-1032 Technical datasheet (24rxns)

15-1032 protocol

Price

£432.00

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