E-64d
Catalogue Number: 15-4009-ABO
| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Protease Inhibitors |
| Alias: | Aloxistatin, E-64c ethyl ester,EP 453, EST, Loxistatin, NSC 694281 |
| Shipping Condition: | Dry Ice |
| Unit(s): | 5 mg, 1 mg |
Description
Description: Molecular Formula: C17H30N2O5 Molecular Weight: 342.4 Cysteine proteases are a class of enzymes containing an active-site cysteine residue that is important in protein degradation pathways. E-64d, a synthetic analog of E-64 and ethyl ester of E-64c, is an irreversible, membrane-permeable inhibitor of lysosomal and cytosolic cysteine proteases.E-64d inhibits calpain and the cysteine proteases cathepsins F, K, B, H, and L. By disrupting protease activity, E-64d, at concentrations between 20-200 µM, has been shown to arrest human epidermoid carcinoma A431 cells at mitotic metaphase.It also inhibits protease-resistant prion protein accumulation in scrapie-infected neuroblastoma cells with an IC50 value of 0.5 µM.
Additional Text
Application Notes
A stock solution may be made by dissolving the E-64d in the solvent of choice. E-64d is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of E-64d in ethanol is approximately 10 mg/ml and approximately 30 mg/ml in DMSO and DMF. E-64d is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, E-64d should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. E-64d has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
15-4009 Datasheet
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