Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence*
Catalogue Number: 15295-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Cell based assay |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
Description
Description: The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. In general, the redox couples NAD/NADH and NADP/NADPH play a critical role in energy metabolism, glycolysis, tricarboxylic acid cycle and mitochondrial respiration. The increased NAD(P)H level in cells is linked to the abnormal production of reactive oxygen species (ROS) and DNA damage. However, due to the lack of sensitive NAD(P)H probe, it has been challenging to detect intracellular NAD(P)H in biological systems. Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit provides an efficient method to monitor intracellular NAD(P)H level in live cells in the far spectrum and can be combined with other applications such as GFP-expressed cells or application of MitoTracker. JJ1902 NAD(P)H sensor has been developed as an excellent fluorescent probe for detecting and imaging NADH/NADPH in cells. The probe which is fluorogenic in nature, binds NADH/NADPH to generate strong fluorescence signal with high sensitivity and specificity. JJ1902 NAD(P)H sensor can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Cy5®. This kit is optimized for fluorescence imaging and microplate reader applications.
Additional Text
Storage Note
Multiple;|6 months
15295 MSDS
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