Mononucleosomes (H2AX), Human Recombinant Biotinylated

Catalogue Number: 16-0013-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Dry Ice
Unit(s): 50 ug

Description

Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AX, H2B, H3 and H4. Accession numbers: H2AX-P16104; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5' biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1.

Additional Text

Application Notes

Mononucleosomes (H2AX), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. EpiCypher Mononucleosomes (H2AX), Human Recombinant Biotinylated do not contain free DNA which could alter assayed activities.