Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated
Catalogue Number: 16-0014-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AZ.1, H2B, H3 and H4. Accession numbers: H2AZ.1-P0C0S5; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5' biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AZ.1 and H2AZ.2 are variants of histone H2A and implicated in diverse cellular functions ranging from transcriptional regulation, chromosome transmission and DNA damage repair. H2AZ has a dedicated deposition machinery (the SWR-C ATPase).
Additional Text
Application Notes
Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. EpiCypher Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated do not contain free DNA which could alter assayed activities.
Storage Note
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0014 Datasheet
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