Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated

Catalogue Number: 16-0014-EPC

Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Dry Ice
Unit(s): 50 ug


Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AZ.1, H2B, H3 and H4. Accession numbers: H2AZ.1-P0C0S5; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5' biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AZ.1 and H2AZ.2 are variants of histone H2A and implicated in diverse cellular functions ranging from transcriptional regulation, chromosome transmission and DNA damage repair. H2AZ has a dedicated deposition machinery (the SWR-C ATPase).

Additional Text

Application Notes

Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. EpiCypher Mononucleosomes (H2AZ.1), Human Recombinant Biotinylated do not contain free DNA which could alter assayed activities.