Mononucleosomes (H3.1ΔN2), Recombinant Human, Biotinylated

Catalogue Number: 16-0023-EPC

Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Dry Ice
Unit(s): 50 ug


Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1ΔN2, and H4 Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H3 beginning with threonine 3, wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.

Additional Text

Application Notes

Mononucleosomes (H3.1ΔN2), Human Recombinant Biotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The N-terminal deletion allows for the study of the role of the N-terminus in many aspects of chromatin biology.