Nucleosome, Recombinant Human, Acidic Patch Mutant H2BE105A,E113A Biotinylated
Catalogue Number: 16-0031-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant mononucleosomes consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence containing a 5' biotin-TEG group has high affinity for histone octamers and is useful for nucleosome assembly [1]. Histone H2B contains a glutamate-to-alanine (E-to-A) substitution at positions 105 and 113 (H2BE105A, E113A). H2BE105 and H2BE113 are among key residues forming a negatively charged region on the nucleosome surface named the "acidic patch". The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2B 105 and E113 both reside in H2B alphaC extension and are associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, HMGN2, and SMARCB1 [2,5].
Additional Text
Application Notes
H2BE105A,E113A mononucleosome is highly purified and suitable for a variety of applications to test the effect of acidic patch mutations on enzymatic activity or chromatin binding. The biotinylated DNA enables affinity binding applications.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-0031 Datasheet
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