H3K9me3 Recombinant Nucleosome, Biotinylated
Catalogue Number: 16-0315-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug, 20 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group. *H3.2K9me3 has a Cys to Ala substitution at position 110.
Additional Text
Modification
Trimethylated, Lys9
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
H3K9me3 nucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. For a corresponding unmodified control, we recommend EpiCypher 16-0006.
Preparation
frozen liquid in a screw cap plastic vial
16-0315 Datasheet
SUPPORT
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DELIVERY
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