Nucleosome, Recombinant Human, H3K9me1 dNuc, Biotinylated

Catalogue Number: 16-0325-EPC

Shelf Life:6 months
Type:DNA Binding Proteins
Shipping Condition:Dry Ice
Unit(s): 50 ug


Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains monomethyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. * H3.2 K9me1 has a Cys to Ala substitution at position 110.

Additional Text


Monomethylated, Lys9

Application Notes

Nucleosome, Recombinant Human, H3K9me1 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K9me1 from EpiCypher contains no free DNA which could alter assayed activities.