Catalogue Number: 16-0325-EPC
|Shelf Life:||6 months|
|Type:||DNA Binding Proteins|
|Shipping Condition:||Dry Ice|
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains monomethyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. * H3.2 K9me1 has a Cys to Ala substitution at position 110.
Nucleosome, Recombinant Human, H3K9me1 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K9me1 from EpiCypher contains no free DNA which could alter assayed activities.