Nucleosome, Recombinant Human, H3K4me2 dNuc, Biotinylated

Catalogue Number: 16-0334-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Nucleosome
Shipping Condition:Dry Ice
Unit(s): 50 ug, 20 ug

Description

Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains dimethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group. *H3.2K4me2 has a Cys to Ala substitution at position 110.

Additional Text

Modification

Dimethylated, Lys4

Application Notes

H3R4me2 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.