Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc, Biotinylated
Catalogue Number: 16-0335-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethyl-lysine at position 4, and acetyl-lysine at positions 9, 14, and 18. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group. * H3K4me3, K9,14,18ac also has a Cys to Ala substitution at position 110.
Additional Text
Modification
Acetylated, Lys9; Trimethylated, Lys4
Application Notes
Nucleosome, Recombinant Human, H3K4me3, K9,14,18ac dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K4me3, K9,14,18ac dNucs from EpiCypher does not contain free DNA which could alter assayed activities.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0335 Datasheet
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