Catalogue Number: 16-0344-EPC
|Shelf Life:||6 months|
|Type:||DNA Binding Proteins|
|Shipping Condition:||Dry Ice|
Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 36, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.
Mononucleosomes (H3.3K36M), Recombinant Human are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. Mononucleosomes (H3.3K36M), Recombinant Human from EpiCypher do not contain free DNA which could alter assayed activities.