Oncogenic Mutant H3.3G34R Recombinant Nucleosome, Biotinylated
Catalogue Number: 16-0346-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: H3.3 is a histone variant found in regions of high chromatin turnover outside of S-phase (e.g., at actively transcribed genes). The substitution of R for G at position 34 of H3.3 interferes with SETD2-mediated H3K36 methylation. This mutation has been associated with bone cancers and astrocytomas [1]. Oncogenic Mutant H3.3G34R Recombinant Nucleosome, Biotinylated consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.3G34R, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [2], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group.
Additional Text
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
Application Notes
H3.3G34R mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. For a corresponding unmodified control, we recommend EpiCypher 16-0006.
16-0346 Datasheet
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