Catalogue Number: 16-0346-EPC
|Shelf Life:||6 months|
|Type:||DNA Binding Proteins|
|Shipping Condition:||Dry Ice|
Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing an arginine at position 34, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of R for G at position 34 in human H3.1 and H3.3 is a dominant negative inhibitor of the EZH2 methytransferase complex PRC2 and associated with pediatric brain cancer (DIPG). The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.
Mononucleosomes (H3.3G34R), Recombinant Human are highly purified and are suitable for use as substrates in enzyme screening assays, structural studies or for effector protein binding experiments. Mononucleosomes (H3.3G34R), Recombinant Human from EpiCypher do not contain free DNA which could alter assayed activities.