Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Biotinylated
Catalogue Number: 16-0362-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains citrulline (instead of arginine) at positions 2, 8, and 17. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group. *H3 also has a Cys to Ala substitution at position 110.
Additional Text
Application Notes
H3Cit2,8,17 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Preparation
50 microgram nucleosome in frozen liquid in a screw cap plastic vial
16-0362 Datasheet
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